Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 320208 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#320208, RRID:AB_492982
- Product name
- PE anti-human FOXP3
- Antibody type
- Monoclonal
- Antigen
- full-length FOXP3 protein
- Description
- FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The FOXP3 antibody 259D recognizes human FOXP3 epitope in the region of amino acids 105-235.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Type 1 diabetes-associated IL2RA variation lowers IL-2 signaling and contributes to diminished CD4+CD25+ regulatory T cell function.
Foxp3-mediated suppression of CD95L expression confers resistance to activation-induced cell death in regulatory T cells.
Identification of IL-17-producing FOXP3+ regulatory T cells in humans.
Insulin-like growth factor-binding protein-2 is a target for the immunomodulation of breast cancer.
Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype.
Intratumoral CD4+CD25+ regulatory T-cell-mediated suppression of infiltrating CD4+ T cells in B-cell non-Hodgkin lymphoma.
Fas-ligand-mediated paracrine T cell regulation by the receptor NKG2D in tumor immunity.
Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.
Garg G, Tyler JR, Yang JH, Cutler AJ, Downes K, Pekalski M, Bell GL, Nutland S, Peakman M, Todd JA, Wicker LS, Tree TI
Journal of immunology (Baltimore, Md. : 1950) 2012 May 1;188(9):4644-53
Journal of immunology (Baltimore, Md. : 1950) 2012 May 1;188(9):4644-53
Foxp3-mediated suppression of CD95L expression confers resistance to activation-induced cell death in regulatory T cells.
Weiss EM, Schmidt A, Vobis D, Garbi N, Lahl K, Mayer CT, Sparwasser T, Ludwig A, Suri-Payer E, Oberle N, Krammer PH
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 15;187(4):1684-91
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 15;187(4):1684-91
Identification of IL-17-producing FOXP3+ regulatory T cells in humans.
Voo KS, Wang YH, Santori FR, Boggiano C, Wang YH, Arima K, Bover L, Hanabuchi S, Khalili J, Marinova E, Zheng B, Littman DR, Liu YJ
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 24;106(12):4793-8
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 24;106(12):4793-8
Insulin-like growth factor-binding protein-2 is a target for the immunomodulation of breast cancer.
Park KH, Gad E, Goodell V, Dang Y, Wild T, Higgins D, Fintak P, Childs J, Dela Rosa C, Disis ML
Cancer research 2008 Oct 15;68(20):8400-9
Cancer research 2008 Oct 15;68(20):8400-9
Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype.
Tran DQ, Ramsey H, Shevach EM
Blood 2007 Oct 15;110(8):2983-90
Blood 2007 Oct 15;110(8):2983-90
Intratumoral CD4+CD25+ regulatory T-cell-mediated suppression of infiltrating CD4+ T cells in B-cell non-Hodgkin lymphoma.
Yang ZZ, Novak AJ, Stenson MJ, Witzig TE, Ansell SM
Blood 2006 May 1;107(9):3639-46
Blood 2006 May 1;107(9):3639-46
Fas-ligand-mediated paracrine T cell regulation by the receptor NKG2D in tumor immunity.
Groh V, Smythe K, Dai Z, Spies T
Nature immunology 2006 Jul;7(7):755-62
Nature immunology 2006 Jul;7(7):755-62
Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.
Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 25;103(17):6659-64
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 25;103(17):6659-64
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes surface stained with CD4 FITC, then intracellular stained with 259D PE. Quadrant markers were set based on staining with PE mouse IgG1, κ isotype control
- Conjugate
- Yellow dye