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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
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- Product number
- 320216 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#320216, RRID:AB_2104902
- Product name
- Pacific Blue™ anti-human FOXP3 [259D]
- Antibody type
- Monoclonal
- Antigen
- full-length FOXP3 protein
- Description
- FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 259D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Blue dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype.
Intratumoral CD4+CD25+ regulatory T-cell-mediated suppression of infiltrating CD4+ T cells in B-cell non-Hodgkin lymphoma.
Fas-ligand-mediated paracrine T cell regulation by the receptor NKG2D in tumor immunity.
Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.
Tran DQ, Ramsey H, Shevach EM
Blood 2007 Oct 15;110(8):2983-90
Blood 2007 Oct 15;110(8):2983-90
Intratumoral CD4+CD25+ regulatory T-cell-mediated suppression of infiltrating CD4+ T cells in B-cell non-Hodgkin lymphoma.
Yang ZZ, Novak AJ, Stenson MJ, Witzig TE, Ansell SM
Blood 2006 May 1;107(9):3639-46
Blood 2006 May 1;107(9):3639-46
Fas-ligand-mediated paracrine T cell regulation by the receptor NKG2D in tumor immunity.
Groh V, Smythe K, Dai Z, Spies T
Nature immunology 2006 Jul;7(7):755-62
Nature immunology 2006 Jul;7(7):755-62
Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development.
Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 25;103(17):6659-64
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 25;103(17):6659-64
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained with CD4 APC and then intracellular stained with Pacific Blue™ anti-human FOXP3 (clone 259D). The quadrant markers were set based on Pacific Blue™ mIgG1, k isotype control
- Conjugate
- Blue dye
