PA1-16876

antibody from Invitrogen Antibodies

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Antibody data

Submit

Validation data

Product number: PA1-16876 - Provider product page
Provider: Invitrogen Antibodies
Product name: FOXP3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
Concentration: 1 mg/mL

FOXP3 protein structure - PA1-16876 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of human FOXP3 using Product # PA1-16876. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FOXP3 in human CD4+CD25+ PBL, HEK293T transfected with human Foxp3 cDNA, 293/mouse foxp3, 293/empty vector. Samples were incubated in FOXP3 polyclonal antibody (Product # PA1-16876). Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-FOXP3 Polyclonal Antibody (Product # PA1-16876) and a 43 kDa band corresponding to FOXP3 was observed in Mouse Thymus in comparison to Mouse Liver, Mouse Kidney and Mouse Skeletal Muscle which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Thymus (Lane 1), Mouse Liver (Lane 2), Mouse Kidney (Lane 3) and Mouse Skeletal Muscle (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of FOXP3 in healthy thymus from a C57BL/6 mouse. Frozen samples were incubated in FOXP3 polyclonal antibody (Product # PA1-16876).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of FOXP3 in formalin-fixed paraffin-embedded tissue sections of human breast cancer. Samples were incubated in FOXP3 polyclonal antibody (Product # PA1-16876) using a dilution of 1:100. ACDs Integrated Co-Detection Workflow was performed using ACD RNAScope Probe. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue). CD8A mRNA (red) and FOXP3 protein (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of FOXP3 in formalin-fixed paraffin-embedded tissue sections of human breast cancer. Samples were incubated in FOXP3 polyclonal antibody (Product # PA1-16876) using a dilution of 1:200. ACDs Integrated Co-Detection Workflow was performed using ACD RNAScope Probe. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue). CD4 mRNA (red) and FOXP3 protein (green).