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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [2]
- Immunohistochemistry [1]
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- Product number
- H00058494-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00058494-M01, RRID:AB_539969
- Product name
- Mouse Monoclonal JAM-B/VE-JAM Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. JAM2 - junctional adhesion molecule 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 expression in LNCaP ( Cat # L004V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Analysis of JAM2 expression in transfected 293T cell line by JAM2 monoclonal antibody (M01), clone 1G4.Lane 1: JAM2 transfected lysate (Predicted MW: 33.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 monoclonal antibody (M01), clone 1G4. Analysis of JAM2 expression in HL-60.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Detection limit for recombinant GST tagged JAM2 is approximately 3ng/ml as a capture antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Detection limit for recombinant GST tagged JAM2 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Analysis of monoclonal antibody to JAM2 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.
