Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10742-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human JAM-B/VE-JAM Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human JAM-B/VE-JAM in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P57087
- Isotype
- IgG
- Antibody clone number
- 988901
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of JAM-B in HEK293 Human Cell Line Transfected with JAM-B and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human JAM-B or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human JAM-B Monoclonal Antibody (Catalog # MAB10742) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.