Explore
Validate
LearnMA5-15583
antibody from Invitrogen Antibodies
Targeting: SMC1A
DXS423E, KIAA0178, SB1.8, SMC1L1, Smcb
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-15583 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMC1 Monoclonal Antibody (5B6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15583 targets SMC1 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 5B6
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SMC1 was achieved by transfecting MOLT-4 with SMC1 specific siRNAs (Silencer® select Product # s15751 and s15752). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the SMC1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMC1 Monoclonal Antibody (5B6) (Product # MA5-15583, 1:1500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMC1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SMC1 Monoclonal Antibody (5B6) (Product # MA5-15583) and a 160 kDa band corresponding to SMC1 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), MOLT-4 (Lane 2), Jurkat (Lane 3), SK-O-V3 (Lane 4), U-87 MG (Lane 5), Hep G2 (Lane 6) and K-562 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMC1 using SMC1 monoclonal antibody (Product # MA5-15583) in K562 (1), Jurkat (2) and A549 (3) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using SMC1 monoclonal antibody (Product # MA5-15583) (Green). Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon using SMC1 monoclonal antibody (Product # MA5-15583) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using SMC1 monoclonal antibody (Product # MA5-15583) (green) and negative control (purple).
