MA5-15623

antibody from Invitrogen Antibodies

Targeting: SYP MRX96

Antibody data

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Validation data

Product number: MA5-15623 - Provider product page
Provider: Invitrogen Antibodies
Product name: Synaptophysin Monoclonal Antibody (7H12)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15623 targets SYP in IHC and WB applications and shows reactivity with Human and Rat samples.
Antibody clone number: 7H12
Concentration: Conc. Not Determined

SYP protein structure - MA5-15623 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Synaptophysin Monoclonal Antibody (7H12) (Product # MA5-15623) and a 38kDa band corresponding to Synaptophysin was observed across in IMR-32, PC-12, Neuro-2a, Mouse Brain and Rat Brain, but not in SK-BR-3, BJ, NIH-3T3, Mouse Kidney and Rat Kidney. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), SK-BR-3 (Lane 2), BJ (Lane 3), PC-12 (Lane 4), Neuro-2a (Lane 5), NIH-3T3 (Lane 6), Mouse Brain (Lane 7), Mouse Kidney (Lane 8), Rat Brain (Lane 9), Rat Kidney (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Synaptophysin Monoclonal Antibody (7H12) (Product # MA5-15623) and a 38kDa band corresponding to Synaptophysin was observed across in IMR-32, PC-12, Neuro-2a, Mouse Brain and Rat Brain, but not in SK-BR-3, BJ, NIH-3T3, Mouse Kidney and Rat Kidney. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), SK-BR-3 (Lane 2), BJ (Lane 3), PC-12 (Lane 4), Neuro-2a (Lane 5), NIH-3T3 (Lane 6), Mouse Brain (Lane 7), Mouse Kidney (Lane 8), Rat Brain (Lane 9), Rat Kidney (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SYP using SYP monoclonal antibody (Product # MA5-15623) in rat brain tissue lysate.