PA5-92879

antibody from Invitrogen Antibodies

Targeting: NEU1 NEU

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92879

Provider

Invitrogen Antibodies

Product name

NEU1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PRKGRLIVCG HGTLERDGVF CLLSDDHGAS WRYGSGVSGI PYGQPKQEND FNPDECQPYE LPDGSVVINA RNQNNYHCHC RIVLRSYDAC DTLRPRDVTF DPELVDPVVA AGAVVTSSGI VFFSNPAHPE FRVNLTLRWS FSNGTSWRKE TVQLWPGPSG YSSLATLEGS MDGEEQAPQL YVLYEKGRNH YTESISVAKI SVYGTL

Concentration

1.29 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using NEU1 Polyclonal antibody (Product # PA5-92879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 3s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NEU1 in extracts of various cell lines using NEU1 Polyclonal Antibody (Product # PA5-92879) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NEU1 in paraffin-embedded mouse spinal cord using NEU1 Polyclonal Antibody (Product # PA5-92879) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NEU1 in paraffin-embedded rat brain using NEU1 Polyclonal Antibody (Product # PA5-92879) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NEU1 in paraffin-embedded mouse spinal cord using NEU1 Polyclonal Antibody (Product # PA5-92879) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NEU1 in paraffin-embedded mouse brain using NEU1 Polyclonal Antibody (Product # PA5-92879) at a dilution of 1:200.