PAB27914

antibody from Abnova Corporation

Targeting: TIMM29 C19orf52, TIM29

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27914

Provider

Abnova Corporation

Product name

C19orf52 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C19orf52.

Antigen sequence

LDVGFVGRWWVLGAWMRDCDINDDEFLHLPAHLRV
VGPQQLHSETNERLFDEKYKPVVLTDDQVDQALWE
EQVLQKEKKD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with C19orf52 polyclonal antibody (Cat # PAB27914) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with C19orf52 polyclonal antibody (Cat # PAB27914) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach, lower with C19orf52 polyclonal antibody (Cat # PAB27914) shows strong nuclear positivity in glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)