PA1-32079

antibody from Invitrogen Antibodies

Targeting: ATP6AP2 APT6M8-9, ATP6IP2, ATP6M8-9, M8-9, PRR, RENR

Immunohistochemistry

Antibody data

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Validation data

Product number: PA1-32079 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATP6IP2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX25959-PEP)..
Reactivity: Human, Mouse, Rat
Host: Goat
Isotype: IgG
Vial size: 50 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ATP6AP2 protein structure - PA1-32079 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATP6IP2 in human Kidney lysate (RIPA buffer 35 µg total protein per lane) using an ATP6IP2 polyclonal antibody (Product # PA1-32079) at a dilution of 0.5 µg/mL following incubation for 1 hour and detected using chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ATP6IP2 was performed by loading 35 µg (in RIPA buffer) of mouse kidney lysates. Proteins were transferred to a membrane and probed with a ATP6IP2 Polyclonal Antibody (Product # PA1-32079) at a dilution of 2 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of ATP6IP2 was achieved by transfecting MCF-7 cells with ATP6IP2 specific siRNAs (Silencer® select Product # s19791, s19790). Western blot analysis (Fig. a) was performed using whole cell extracts from the MCF-7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ATP6IP2 Polyclonal Antibody (Product # PA1-32079, 0.125 µg/mL) and Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b, Fig.c). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP6IP2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ATP6IP2 Polyclonal Antibody (Product # PA1-32079) and ~37 kDa band corresponding to ATP6IP2 was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), PC-3 (Lane 3), SiHa (Lane 4), Panc-1 (Lane 5) and HCT 116 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).