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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34686 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Biglycan Recombinant Rabbit Monoclonal Antibody (JB71-31)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HepG2, human kidney tissue, mouse heart tissue, human skin tissue lysates.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB71-31
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Biglycan in mouse heart and human skin tissue lysates. Samples were incubated with Biglycan monoclonal antibody (Product # MA5-34686), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Biglycan in paraffin-embedded mouse heart tissue. Samples were incubated with Biglycan monoclonal antibody (Product # MA5-34686), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Biglycan in paraffin-embedded human kidney tissue. Samples were incubated with Biglycan monoclonal antibody (Product # MA5-34686), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Biglycan in HepG2 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Biglycan monoclonal antibody (Product # MA5-34686) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
