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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 701265 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Insulin Recombinant Rabbit Monoclonal Antibody (19H4L12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Antibody clone number
- 19H4L12
- Concentration
- 0.5 mg/mL
Submitted references Pancreatic Ppy-expressing γ-cells display mixed phenotypic traits and the adaptive plasticity to engage insulin production.
Perez-Frances M, van Gurp L, Abate MV, Cigliola V, Furuyama K, Bru-Tari E, Oropeza D, Carreaux T, Fujitani Y, Thorel F, Herrera PL
Nature communications 2021 Jul 22;12(1):4458
Nature communications 2021 Jul 22;12(1):4458
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Insulin in human pancreatic tissue using an Insulin recombinant rabbit monoclonal antibody (Product # 701265) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) and nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Insulin in human pancreatic tissue using an Insulin monoclonal antibody (Product # 701265) followed by detection using an HRP-conjugated goat anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Adult gamma-cells derive from embryonic Ppy-expressing cells. a Embryonic Ppy-expressing cell labeling strategy. DOX doxycycline, P30 postnatal day 30. b The proportion of adult YFP-labeled Ppy-expressing cells is maintained in the different DOX administration periods. DOX E7.5 to P30, analysis P30 (black; n = 4 mice, 1842 Ppy+YFP+ out of 2131 Ppy+ cells scored); DOX E7.5 to E19.5, analysis P30 (dark gray, n = 7 mice, 3111 Ppy+YFP+ out of 3604 Ppy+ cells scored); DOX E7.5 to E19.5, analysis 9-month-old (light gray, n = 4 mice, 2241 Ppy+YFP+ out of 2565 Ppy+ cells scored). Data are shown as mean +- s.e.m; two-tailed Mann-Whitney test. P values: DOX E7.5 to P30, analysis P30 vs DOX E7.5 to E19.5, analysis P30 = 0.9273; DOX E7.5 to P30, analysis P30 vs DOX E7.5 to E19.5, analysis long term = 0.6857. c Percentage of adult hormone + cells (Gcg + , Sst + or Ins + ) labeled with YFP when DOX was given during embryogenesis. 21.1 +- 0.0%, 10.8 +- 0.0% and 6.1 +- 0.0% of Gcg + , Sst + and Ins + , respectively, were YFP-traced. The percentage of YFP-labeled Ppy+ cells is taken for reference from panel b . Data are shown as mean +- s.e.m; n = 7 mice; 810 YFP+Gcg+ out of 4196 Gcg+, 285 YFP+Sst+ out of 2669 Sst+ and 1306 YFP+Ins+ out of 20753 Ins+ cells scored. d Immunofluorescence of YFP (green)-traced cells co-expressing Glucagon (red, left panel), Somatostatin (red, middle panel) and Insulin (red, right panel) with Ppy (cyan) at P30. Scale bar: 20 mum (10 mum in insets). Region o
