Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [5]
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- Product number
- 11-1239-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD123 Monoclonal Antibody (6H6), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
- Conjugate
- Green dye
- Antibody clone number
- 6H6
- Concentration
- 5 µL/Test
Submitted references Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.
Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium.
Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.
Group 2 innate lymphoid cell proportions are diminished in young helminth infected children and restored by curative anti-helminthic treatment.
Distinct parameters of the basophil activation test reflect the severity and threshold of allergic reactions to peanut.
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.
The activation pattern of blood leukocytes in head and neck squamous cell carcinoma is correlated to survival.
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.
Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.
Local activation of the innate immune system in Buruli ulcer lesions.
Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.
Baumgaertner P, Sankar M, Herrera F, Benedetti F, Barras D, Thierry AC, Dangaj D, Kandalaft LE, Coukos G, Xenarios I, Guex N, Harari A
Frontiers in immunology 2021;12:633910
Frontiers in immunology 2021;12:633910
Biological Therapy in Inflammatory Bowel Disease Patients Partly Restores Intestinal Innate Lymphoid Cell Subtype Equilibrium.
Creyns B, Jacobs I, Verstockt B, Cremer J, Ballet V, Vandecasteele R, Vanuytsel T, Ferrante M, Vermeire S, Van Assche G, Ceuppens JL, Breynaert C
Frontiers in immunology 2020;11:1847
Frontiers in immunology 2020;11:1847
Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.
Bastian OW, Croes M, Alblas J, Koenderman L, Leenen LPH, Blokhuis TJ
Frontiers in immunology 2018;9:945
Frontiers in immunology 2018;9:945
Group 2 innate lymphoid cell proportions are diminished in young helminth infected children and restored by curative anti-helminthic treatment.
Nausch N, Appleby LJ, Sparks AM, Midzi N, Mduluza T, Mutapi F
PLoS neglected tropical diseases 2015 Mar;9(3):e0003627
PLoS neglected tropical diseases 2015 Mar;9(3):e0003627
Distinct parameters of the basophil activation test reflect the severity and threshold of allergic reactions to peanut.
Santos AF, Du Toit G, Douiri A, Radulovic S, Stephens A, Turcanu V, Lack G
The Journal of allergy and clinical immunology 2015 Jan;135(1):179-86
The Journal of allergy and clinical immunology 2015 Jan;135(1):179-86
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
Ataide MA, Andrade WA, Zamboni DS, Wang D, Souza Mdo C, Franklin BS, Elian S, Martins FS, Pereira D, Reed G, Fitzgerald KA, Golenbock DT, Gazzinelli RT
PLoS pathogens 2014 Jan;10(1):e1003885
PLoS pathogens 2014 Jan;10(1):e1003885
Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.
Compté N, Zouaoui Boudjeltia K, Vanhaeverbeek M, De Breucker S, Tassignon J, Trelcat A, Pepersack T, Goriely S
PloS one 2013;8(6):e65325
PloS one 2013;8(6):e65325
The activation pattern of blood leukocytes in head and neck squamous cell carcinoma is correlated to survival.
Millrud CR, Månsson Kvarnhammar A, Uddman R, Björnsson S, Riesbeck K, Cardell LO
PloS one 2012;7(12):e51120
PloS one 2012;7(12):e51120
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Landrigan A, Wong MT, Utz PJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.
Assaf C, Gellrich S, Whittaker S, Robson A, Cerroni L, Massone C, Kerl H, Rose C, Chott A, Chimenti S, Hallermann C, Petrella T, Wechsler J, Bagot M, Hummel M, Bullani-Kerl K, Bekkenk MW, Kempf W, Meijer CJ, Willemze R, Sterry W
Journal of clinical pathology 2007 Sep;60(9):981-9
Journal of clinical pathology 2007 Sep;60(9):981-9
Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.
Xu W, He B, Chiu A, Chadburn A, Shan M, Buldys M, Ding A, Knowles DM, Santini PA, Cerutti A
Nature immunology 2007 Mar;8(3):294-303
Nature immunology 2007 Mar;8(3):294-303
Local activation of the innate immune system in Buruli ulcer lesions.
Peduzzi E, Groeper C, Schütte D, Zajac P, Rondini S, Mensah-Quainoo E, Spagnoli GC, Pluschke G, Daubenberger CA
The Journal of investigative dermatology 2007 Mar;127(3):638-45
The Journal of investigative dermatology 2007 Mar;127(3):638-45
Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.
Jaye DL, Geigerman CM, Herling M, Eastburn K, Waller EK, Jones D
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Dec;19(12):1555-62
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Dec;19(12):1555-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) APC (Product # 17-5179-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD123 FITC (right). Cells in the monocyte gate and lymphocyte were used for analysis.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
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- Invitrogen Antibodies (provider)
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- Submitted by
- Invitrogen Antibodies (provider)
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Identification of multiple mDC and pDC subsets by MegaClust. (A) Comparison of the different clusters identified by MegaClust as mDCs and pDCs (example for one patient for four time points). (B) Expression level of CD123 and CD11c of the two mDC clusters and the two pDC clusters identified by MegaClust. (C) Expression levels of HLA-DR and CD4 for the two pDC clusters and the two mDC clusters identified by MegaClust. Cluster 53 could be assigned as basophils due to the lack of expression of CD4 and HLA-DR (). The expression of CD4 is shown for CD4 T cells and CD14 + monocyte clusters for comparison. (D) Overlay of CD4 and HLA-DR of MegaClust identified clusters for mDC, pDC, basophils, CD4 and monocytes. (E) mDCs and pDCs were discriminated according to CD11c and CD123 expression in the supervised flow cytometry re-analysis according to the new gating strategy described in Supplementary Figure 5 (one representative patient 0QZW). (F) Representative illustration of HLA-DR and CD4 co-expression on mDCs and pDCs prior to FACS sorting CD11c+CD123 - CD4 + HLA-DR+ mDCs and CD123 + CD11c - CD4 + HLA-DR + were FACS sorted (dotted lines). (G) Gene expression profiles of flow cytometry sorted CD11c + CD123 - CD4 + HLA-DR + mDCs, CD123 + CD11c - CD4 + HLA-DR + CD4 + HLA-DR + pDC and CD14 + CD16 - monocytes from 3 healthy donors are shown in comparison to the RNA expression profile of public available RNAseq datasets (Immgen) indicating the gene expression profile of B cells, mac
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- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 (A) Surface antigen expression of bone marrow stromal cells (BMSCs) isolated from the talus bone marrow using flow cytometry. >95% of cells were negative for CD45 and CD14, and >99% of cells were negative for CD19 and CD34. In addition, >95% were positive for CD73, CD90, CD105, and CD140b. Since plastic adherence is a well-established and validated technique to isolate multipotent stromal cells (MSCs), we have only characterized one BMSC donor using flowcytometry instead of all donors. The blue lines are stained cells and the gray lines are negative (unstained) controls. Adapted from Croes et al. ( 25 ). (B) Fluorescence-activated cell sorting (FACS) gating strategy used to isolate granulocytes/neutrophils from peripheral blood leukocytes. Granulocytes were either isolated from unlabeled leukocytes using gate 1 (G1) within the forward/sideward scatter (FSC/SSC). Alternatively, leukocytes were stained using CD3, CD14, CD193, and CD123. Within the FSC/SSC of these labeled cells, debris was first excluded [gate 2 (G2)]. Subsequently, CD3+ cells (lymphocytes) and CD14+ (monocytes) were excluded [gate 3 (G3)]. In addition, CD193+ cells (eosinophils) and CD123+ cells (basophils) were excluded [gate 4 (G4)]. The remaining CD3- CD14- CD193- CD123- cells were defined FACS-sorted neutrophils (G2+, G3+, G4+ sorted neutrophils). Re-analysis of FACS-sorted neutrophils shows adequate exclusion of lymphocytes and monocytes based on their FSC/SSC. (C) Images of BMSCs obtained by arr
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