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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations [0]
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- Product number
- 13-1239-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD123 Monoclonal Antibody (6H6), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. Applications Reported: The 6H6 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 6H6 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 6H6
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.
Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.
PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.
CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.
Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.
Local activation of the innate immune system in Buruli ulcer lesions.
Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.
Bastian OW, Croes M, Alblas J, Koenderman L, Leenen LPH, Blokhuis TJ
Frontiers in immunology 2018;9:945
Frontiers in immunology 2018;9:945
Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.
Lim AI, Li Y, Lopez-Lastra S, Stadhouders R, Paul F, Casrouge A, Serafini N, Puel A, Bustamante J, Surace L, Masse-Ranson G, David E, Strick-Marchand H, Le Bourhis L, Cocchi R, Topazio D, Graziano P, Muscarella LA, Rogge L, Norel X, Sallenave JM, Allez M, Graf T, Hendriks RW, Casanova JL, Amit I, Yssel H, Di Santo JP
Cell 2017 Mar 9;168(6):1086-1100.e10
Cell 2017 Mar 9;168(6):1086-1100.e10
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
Ataide MA, Andrade WA, Zamboni DS, Wang D, Souza Mdo C, Franklin BS, Elian S, Martins FS, Pereira D, Reed G, Fitzgerald KA, Golenbock DT, Gazzinelli RT
PLoS pathogens 2014 Jan;10(1):e1003885
PLoS pathogens 2014 Jan;10(1):e1003885
Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.
Compté N, Zouaoui Boudjeltia K, Vanhaeverbeek M, De Breucker S, Tassignon J, Trelcat A, Pepersack T, Goriely S
PloS one 2013;8(6):e65325
PloS one 2013;8(6):e65325
PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.
van de Laar L, van den Bosch A, Boonstra A, Binda RS, Buitenhuis M, Janssen HL, Coffer PJ, Woltman AM
Blood 2012 Dec 13;120(25):4982-91
Blood 2012 Dec 13;120(25):4982-91
CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.
Assaf C, Gellrich S, Whittaker S, Robson A, Cerroni L, Massone C, Kerl H, Rose C, Chott A, Chimenti S, Hallermann C, Petrella T, Wechsler J, Bagot M, Hummel M, Bullani-Kerl K, Bekkenk MW, Kempf W, Meijer CJ, Willemze R, Sterry W
Journal of clinical pathology 2007 Sep;60(9):981-9
Journal of clinical pathology 2007 Sep;60(9):981-9
Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.
Xu W, He B, Chiu A, Chadburn A, Shan M, Buldys M, Ding A, Knowles DM, Santini PA, Cerutti A
Nature immunology 2007 Mar;8(3):294-303
Nature immunology 2007 Mar;8(3):294-303
Local activation of the innate immune system in Buruli ulcer lesions.
Peduzzi E, Groeper C, Schütte D, Zajac P, Rondini S, Mensah-Quainoo E, Spagnoli GC, Pluschke G, Daubenberger CA
The Journal of investigative dermatology 2007 Mar;127(3):638-45
The Journal of investigative dermatology 2007 Mar;127(3):638-45
Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.
Jaye DL, Geigerman CM, Herling M, Eastburn K, Waller EK, Jones D
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Dec;19(12):1555-62
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Dec;19(12):1555-62
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