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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB160R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human CXCR3 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P49682- Isotype
- IgG
- Antibody clone number
- 49801R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR3 in Human Tonsil. CXCR3 was detected in perfusion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to infiltrating lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR3 in HEK293 Human Cell Line Transfected with human CXCR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human CXCR3 or (B) irrelevant transfectants and eGFP was stained with and either Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL11/I-TAC and Neutralization by Human CXCR3 Antibody. Recombinant Human CXCL11/I-TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R). The ND50 is typically 0.3-1.5 µg/mL.
