Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC6030G - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Indoleamine 2,3-dioxygenase/IDO Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.
CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
Early growth response-2 signaling mediates immunomodulatory effects of human multipotential stromal cells.
Love AC, Schwartz I, Petzke MM
Journal of leukocyte biology 2015 Feb;97(2):379-90
Journal of leukocyte biology 2015 Feb;97(2):379-90
CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients.
Kaltenmeier C, Gawanbacht A, Beyer T, Lindner S, Trzaska T, van der Merwe JA, Härter G, Grüner B, Fabricius D, Lotfi R, Schwarz K, Schütz C, Hönig M, Schulz A, Kern P, Bommer M, Schrezenmeier H, Kirchhoff F, Jahrsdörfer B
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3768-77
Journal of immunology (Baltimore, Md. : 1950) 2015 Apr 15;194(8):3768-77
Early growth response-2 signaling mediates immunomodulatory effects of human multipotential stromal cells.
Barbeau DJ, La KT, Kim DS, Kerpedjieva SS, Shurin GV, Tamama K
Stem cells and development 2014 Jan 15;23(2):155-66
Stem cells and development 2014 Jan 15;23(2):155-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Indoleamine 2,3-dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6030G) or (B) Mouse IgG1 Alexa Fluor 488 Isotype Control (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.