Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A02386-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-GAB2 (Ab-159) Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody for Q9UQC2 detection. Tested positive for WB IHC in Hu
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100ul
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- Store at -20˚C
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- IHC analysis of GAB2 using anti-GAB2 antibody (A02386-2).GAB2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GAB2 Antibody (A02386-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image