NBP2-25149

antibody from Novus Biologicals

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-25149

Provider

Novus Biologicals

Product name

Mouse Monoclonal HSP27 Antibody

Antibody type

Monoclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.