ADI-SPA-524-D

antibody from Enzo Life Sciences

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

ADI-SPA-524-D

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-SPA-524-D, RRID:AB_2039224

Product name

[pSer82]HSP27 polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Sheep

Host

Rabbit

Vial size

50 μl

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Release of Phosphorylated HSP27 (HSPB1) from Platelets Is Accompanied with the Acceleration of Aggregation in Diabetic Patients.

Tokuda H, Kuroyanagi G, Tsujimoto M, Enomoto Y, Matsushima-Nishiwaki R, Onuma T, Kojima A, Doi T, Tanabe K, Akamatsu S, Iida H, Ogura S, Otsuka T, Iwama T, Tanikawa T, Ishikawa K, Kojima K, Kozawa O
PloS one 2015;10(6):e0128977

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Age-related differences in skeletal muscle insulin signaling: the role of stress kinases and heat shock proteins.

Gupte AA, Bomhoff GL, Geiger PC
Journal of applied physiology (Bethesda, Md. : 1985) 2008 Sep;105(3):839-48

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of phosphorylated HSP27 and nonphosphorylated HSP27 proteins probed with HSP27 (pSer82) pAb

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunofluorescent analysis (confocal) staining of HeLa cells using Hsp27 (pSer82) pAb (green); nuclei are stained in blue pseudocolor using DRAQ5