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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 12-6526-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- B7-H6 Monoclonal Antibody (JAM1EW), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Description: This JAM1EW monoclonal antibody recognizes human B7-H6 (NCR3LG1) protein on the surface of cells. It recognizes protein on live but not on paraformaldehyde-fixed cells. The JAM1EW clone is specific to B7-H6. When tested in flow cytometry against cells transfected with either B7-H4, B7-H5, B7-H6, or B7-H7, clone JAM1EW only stains positively on B7-H6 transfected cells.
- Conjugate
- Yellow dye
- Antibody clone number
- JAM1EW
- Concentration
- 5 µL/Test
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- HeLa cells were stained with Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (blue) or B7-H6 Monoclonal Antibody, PE (purple). Total viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18), were used for analysis.
- Conjugate
- Yellow dye
