PAB19940

antibody from Abnova Corporation

Targeting: PDCD4 H731

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB19940

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB19940, RRID:AB_10961892

Product name

PDCD4 (phospho S457) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDCD4.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of recombinant protein with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940). Lane 1& 2 each contain 100 ng recombinant PDCD4. Lanes 3& 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4)

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of formalin-fixed paraffin-embedded human breast epithelial cell with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)