ABIN2616812

antibody from antibodies-online

Targeting: APEX1 APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2616812

Provider

antibodies-online

Product name

anti-APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) (AA 219-318) antibody

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human

Host

Mouse

Epitope

AA 219-318

Isotype

IgG

Vial size

100 μg

Storage

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Handling

Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys5 antibody tested by Western blot. HeLa acid extract probed with Histone H4 acetyl Lys5 polyclonal antibody (1:1,000 dilution).     Lane 1: No treatment.     Lane 2: Cells treated with sodium butyrate.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys5 antibody specificity data. Top Panel ? Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for H4 acetyl lysine 5. Peptides corresponding to regions around major sites of histone H4 acetylation were spotted onto PVDF and probed at a dilution of 1:1,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide. Bottom Panel ? Peptide inhibition analysis. A Western blot was performed on butyrate-treated HeLa cell acid extract (5 μl per lane) at a dilution of 1:2,000. Different peptides were incubated with the antibody during the blotting procedure to determine if the recognition was blocked by a specific modification. Lane 1: no peptide. Lane 2: acetyl-Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: acetyl-Lys12 peptide. Lane 5: acetyl-Lys16 peptide.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys5 antibody tested by immunofluorescence. Staining of HeLa cells with Histone H4 acetyl Lys5 antibody (1:500 dilution, top panel) and DAPI (bottom panel).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys5 antibody tested by ChIP analysis. Chromatin IP performed on HeLa cell chromatin using 39169. PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: negative IgG control. Lane 2: ChIP using 10 ul of 39169. Lane 3: Input DNA control.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys5 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 μl of Histone H4 acetyl Lys5 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.