ABIN2616819

antibody from antibodies-online

Targeting: APEX1 APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2616819

Provider

antibodies-online

Product name

anti-APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody (HRP)

Antibody type

Polyclonal

Description

Caprylic acid and ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Conjugate

Horseradish Peroxidase

Isotype

IgG

Vial size

100 μg

Storage

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Handling

Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Specificity Data. Top Panel: Dot blot was used to confirm the specificity of Histone H4 acetyl Lys12 antibody for H4 acetyl Lys12. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Histone H4 acetyl-Lys5 peptide. Lane 2: Unmodified Lys5 peptide. Lane 3: Acetyl-Lys8 peptide.Lane 4: Unmodified Lys8 peptide. Lane 5: Acetyl-Lys12 peptide. Lane 6: Unmodified Lys12 peptide. Lane 7: Acetyl-Lys16 peptide. Lane 8: Unmodified Lys16 peptide. Bottom Panel: Western blot (1:2,000 dilution) using whole-cell extracts (35 μg per lane) from wild-type yeast or yeast containing a mutated histone H4 gene. Lane 1: Wild-type H4. Lane 2: H4 N-terminal tail deleted. Lane 3: Histone H4 K5 to R. Lane 4: H4 with K8 to R. Lane 5: H4 with K12 to R. Lane 6: H4 K16 to R. Lane 7: H4 K5, 8 & 12 to R.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys12 antibody tested by Western blot. HeLa acid extract probed with Histone H4 acetyl Lys12 polyclonal antibody (1:10,000 dilution).     Lane 1: No treatment.     Lane 2: Cells treated with sodium butyrate.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys12 antibody tested by immunofluorescence. Staining of HeLa cells with Histone H4 acetyl Lys12 antibody (1:500 dilution, top panel) and DAPI (bottom panel).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H4 acetyl Lys12 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 μl of Histone H4 acetyl Lys12 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.