Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA5-28831 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ephrin B3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, mouse hippocampus, rat hippocampus.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ephrin-B3 using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Ephrin-B3 polyclonal antibody (Product # PA5-28831) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ephrin B3 was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Ephrin B3 Polyclonal Antibody (Product # PA5-28831) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ephrin B3 was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Ephrin B3 Polyclonal Antibody (Product # PA5-28831) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Ephrin B3 Polyclonal Antibody (Product # PA5-28831). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ephrin B3 Polyclonal Antibody (Product # PA5-28831) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primar.