ABIN362124

antibody from antibodies-online

Targeting: FOXO1 FKH1, FKHR, FOXO1A

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362124

Provider

antibodies-online

Product name

anti-Forkhead Box O1 (FOXO1) (pSer319) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer319 (T-S-S (p) -N-A) derived from Human FKHR, FOXO1A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

pSer319

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Two novel phosphorylation sites on FKHR that are critical for its nuclear exclusion.

Rena G, Woods YL, Prescott AR, Peggie M, Unterman TG, Williams MR, Cohen P
The EMBO journal 2002 May 1;21(9):2263-71

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The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site.

Woods YL, Rena G, Morrice N, Barthel A, Becker W, Guo S, Unterman TG, Cohen P
The Biochemical journal 2001 May 1;355(Pt 3):597-607

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Roles of the forkhead in rhabdomyosarcoma (FKHR) phosphorylation sites in regulating 14-3-3 binding, transactivation and nuclear targetting.

Rena G, Prescott AR, Guo S, Cohen P, Unterman TG
The Biochemical journal 2001 Mar 15;354(Pt 3):605-12