Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA500548 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500548, RRID:AB_2120320
- Product name
- Anti-HSPA9 mouse monoclonal antibody, clone OTI4A1 (formerly 4A1)
- Antibody type
- Monoclonal
- Description
- Anti-HSPA9 mouse monoclonal antibody, clone OTI4A1 (formerly 4A1)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HSPA9
- Isotype
- IgG
- Antibody clone number
- OTI4A1
- Vial size
- 100 µl
- Concentration
- 1.1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (RC201397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9 .
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HSPA9 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-HSPA9 mouse monoclonal antibody (TA500548) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 (RC201397).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-HSPA9 mouse monoclonal antibody (TA500548).
- Validation comment
- IF