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LearnNBP1-79019
antibody from Novus Biologicals
Targeting: GLI3
ACLS, GCPS, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-79019 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-79019, RRID:AB_11019866
- Product name
- Rabbit Polyclonal GLI-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Anti-apoptotic role of the sonic hedgehog signaling pathway in the proliferation of ameloblastoma.
Kanda S, Mitsuyasu T, Nakao Y, Kawano S, Goto Y, Matsubara R, Nakamura S
International journal of oncology 2013 Sep;43(3):695-702
International journal of oncology 2013 Sep;43(3):695-702
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GLI-3 Antibody [NBP1-79019] - Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-GLI3 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. GLI3 was also immunoprecipitated by rabbit anti-GLI3 antibody NBP1-79020 which recognizes a downstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP1-79019] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- GLI3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
