Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-17127 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Flotillin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Neuronal uptake of tau/pS422 antibody and reduced progression of tau pathology in a mouse model of Alzheimer's disease.
Collin L, Bohrmann B, Göpfert U, Oroszlan-Szovik K, Ozmen L, Grüninger F
Brain : a journal of neurology 2014 Oct;137(Pt 10):2834-46
Brain : a journal of neurology 2014 Oct;137(Pt 10):2834-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Flotillin-1 in extracts from mouse lung and rat brain using Flotillin-1 polyclonal antibody (Product # PA5-17127).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-Flotillin 1 Polyclonal Antibody (Product # PA5-17127) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), A549 (Lane 6), THP-1 (Lane 7), tissue extracts of Mouse Lung (Lane 8), Mouse Brain (Lane 9) and Mouse Heart (Lane 10) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).