- 44-380G - Invitrogen Antibodies STAT3 antibody

Submitted references βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity.

Ghosh S, Liu H, Yazdankhah M, Stepicheva N, Shang P, Vaidya T, Hose S, Gupta U, Calderon MJ, Hu MW, Nair AP, Weiss J, Fitting CS, Bhutto IA, Gadde SGK, Naik NK, Jaydev C, Lutty GA, Handa JT, Jayagopal A, Qian J, Sahel JA, Rajasundaram D, Sergeev Y, Zigler JS Jr, Sethu S, Watkins S, Ghosh A, Sinha D
Communications biology 2021 Feb 24;4(1):248

RAC3 Promotes Proliferation, Migration and Invasion via PYCR1/JAK/STAT Signaling in Bladder Cancer.

Cheng C, Song D, Wu Y, Liu B
Frontiers in molecular biosciences 2020;7:218

Effects of general anesthesia with or without epidural block on tumor metastasis and mechanisms.

Yang B, Qian F, Li W, Li Y, Han Y
Oncology letters 2018 Apr;15(4):4662-4668

Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis.

Bao YN, Cao X, Luo DH, Sun R, Peng LX, Wang L, Yan YP, Zheng LS, Xie P, Cao Y, Liang YY, Zheng FJ, Huang BJ, Xiang YQ, Lv X, Chen QY, Chen MY, Huang PY, Guo L, Mai HQ, Guo X, Zeng YX, Qian CN
Cell cycle (Georgetown, Tex.) 2014;13(12):1958-69

Polysaccharide isolated from Angelica sinensis inhibits hepcidin expression in rats with iron deficiency anemia.

Liu JY, Zhang Y, You RX, Zeng F, Guo D, Wang KP
Journal of medicinal food 2012 Oct;15(10):923-9

Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression.

Wang KP, Zeng F, Liu JY, Guo D, Zhang Y
Journal of ethnopharmacology 2011 Apr 12;134(3):944-8

A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.

Ding L, Paszkowski-Rogacz M, Nitzsche A, Slabicki MM, Heninger AK, de Vries I, Kittler R, Junqueira M, Shevchenko A, Schulz H, Hubner N, Doss MX, Sachinidis A, Hescheler J, Iacone R, Anastassiadis K, Stewart AF, Pisabarro MT, Caldarelli A, Poser I, Theis M, Buchholz F
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Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen.

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Molecular biology of the cell 2005 Aug;16(8):3832-46

The ribosomal S6 kinases, cAMP-responsive element-binding, and STAT3 proteins are regulated by different leukemia inhibitory factor signaling pathways in mouse embryonic stem cells.

Boeuf H, Merienne K, Jacquot S, Duval D, Zeniou M, Hauss C, Reinhardt B, Huss-Garcia Y, Dierich A, Frank DA, Hanauer A, Kedinger C
The Journal of biological chemistry 2001 Dec 7;276(49):46204-11

Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes.

Stephens JM, Lumpkin SJ, Fishman JB
The Journal of biological chemistry 1998 Nov 20;273(47):31408-16

Activation of signal transducers and activators of transcription 1 and 3 by leukemia inhibitory factor, oncostatin-M, and interferon-gamma in adipocytes.

Stephens JM, Lumpkin SJ, Fishman JB
The Journal of biological chemistry 1998 Nov 20;273(47):31408-16

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: STAT3 (pY705) (human) phosphospecific antibody. 3T3-L1 cells were stimulated to show specific phosphorylation at tyrosine 705 with IFN, LIF, or PDGF stimulation (upper panel). Pan STAT3 (Product # 44-364G) is used to show consistent presence of STAT3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: STAT3 (pY705) (human) phosphospecific antibody. 3T3-L1 cells were stimulated to show specific phosphorylation at tyrosine 705 with IFN, LIF, or PDGF stimulation (upper panel). Pan STAT3 (Product # 44-364G) is used to show consistent presence of STAT3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: STAT3 (pY705) (human) phosphospecific antibody. 3T3-L1 cells were stimulated to show specific phosphorylation at tyrosine 705 with IFN, LIF, or PDGF stimulation (upper panel). Pan STAT3 (Product # 44-364G) is used to show consistent presence of STAT3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Peptide Competition. Extracts of 3T3L1 cells unstimulated (1) or stimulated with 20 ng/mL LIF for 10 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with the STAT3 (pY705) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), a generic phosphotyrosine-containing peptide (3), or the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F (ab’)2 anti-rabbit IgG HRP-conjugate (Product # ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to STAT3 (pY705) blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of STAT3 (pY705) phosphorylation by the addition of LIF in this cell system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

44-380G

Antibody data