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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF4934 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-STAT3 (S727) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human STAT3 when phosphorylated at S727 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P40763- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation.
Saarimäki-Vire J, Balboa D, Russell MA, Saarikettu J, Kinnunen M, Keskitalo S, Malhi A, Valensisi C, Andrus C, Eurola S, Grym H, Ustinov J, Wartiovaara K, Hawkins RD, Silvennoinen O, Varjosalo M, Morgan NG, Otonkoski T
Cell reports 2017 Apr 11;19(2):281-294
Cell reports 2017 Apr 11;19(2):281-294
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Phospho-STAT3 (S727) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-STAT3 (S727) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4934) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT3 (S727) was detected at approximately 110 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human STAT3 Monoclonal Antibody (lower panel, Catalog # MAB1799). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
