Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-9044-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-STAT4 (Tyr693) Monoclonal Antibody (4LURPIE), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 4LURPIE monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 4 (STAT4) when phosphorylated on tyrosine 693 (Y693). STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAK proteins, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT4 is activated by IL-12 or type 1 IFN (IFN alpha, IFN beta). STAT4 is required for optimal Th1 differentiation in vivo. Interestingly, although IFN alpha can lead to phosphorylation of STAT4 in mouse T cells, the phosphorylation is much weaker than that induced by IL-12 and is transient. Thus, IFN alpha phosphorylation of STAT4 does not contribute to STAT4-mediated Th1 development or IFN gamma production by Th1 cells in mice. In contrast, IFN alpha does promote Th1 development and function of human CD4+ T cells.
- Conjugate
- Yellow dye
- Antibody clone number
- 4LURPIE
- Concentration
- 5 µL/Test
Submitted references ILC3 deficiency and generalized ILC abnormalities in DOCK8-deficient patients.
Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity.
Eken A, Cansever M, Okus FZ, Erdem S, Nain E, Azizoglu ZB, Haliloglu Y, Karakukcu M, Ozcan A, Devecioglu O, Aksu G, Arikan Ayyildiz Z, Topal E, Karakoc Aydiner E, Kiykim A, Metin A, Cipe F, Kaya A, Artac H, Reisli I, Guner SN, Uygun V, Karasu G, Dönmez Altuntas H, Canatan H, Oukka M, Ozen A, Chatila TA, Keles S, Baris S, Unal E, Patiroglu T
Allergy 2020 Apr;75(4):921-932
Allergy 2020 Apr;75(4):921-932
Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity.
Cuadrado E, van den Biggelaar M, de Kivit S, Chen YY, Slot M, Doubal I, Meijer A, van Lier RAW, Borst J, Amsen D
Immunity 2018 May 15;48(5):1046-1059.e6
Immunity 2018 May 15;48(5):1046-1059.e6
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of untreated (orange histogram) or 15-minute IFN alpha2c-treated (purple histogram) Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81)-activated human CD4+ T cells with Anti-Human/Mouse phospho-STAT4 (Y693) PE. Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye