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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- R1492 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1492, RRID:AB_1007504
- Product name
- anti STAT4
- Antibody type
- Polyclonal
- Antigen
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 730-749 of Mouse STAT -4. Swiss Prot accession number P42228.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 85 mg/ml (by refractometry)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Figure 1. Western blot following immunoprecipitation of STAT4 using Rabbit-anti-STAT4 antibody. Lane 2 shows the detection of a single band corresponding to mouse STAT4. Molecular weight markers (not shown) confirm a MW of ~ 85 kDa. For IP use approximately 5 µl of antiserum. A 1:5,000 dilution of the antiserum was used for western blotting. Detection occurs using a 1:2,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure approximately 45''. Other detection systems will yield similar results.
