686013

antibody from BioLegend

Targeting: STAT6 D12S1644, IL-4-STAT

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

686013

Provider

BioLegend

Proper citation

BioLegend Cat#686013, RRID:AB_2632686

Product name

PE/Cy7 anti-STAT6 Phospho (Tyr641)

Antibody type

Monoclonal

Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, transclocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Reactivity

Human

Host

Mouse

Conjugate

Near infrared dye

Antibody clone number

A15137E

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with STAT6 Phospho (Tyr 641) (clone A15137E) PE/Cy7.

Conjugate

Near infrared dye