NBP1-84003

antibody from Novus Biologicals

Targeting: NECAB1 EFCBP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-84003

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-84003, RRID:AB_11028449

Product name

Rabbit Polyclonal NECAB1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human NECAB1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NECAB1 Antibody [NBP1-84003] - Analysis in control (vector only transfected HEK293T lysate) and NECAB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NECAB1 Antibody [NBP1-84003] - Analysis NBP1-84003 (A) shows similar pattern to independent antibody NBP1-84004 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human hippocampus shows moderate cyoplasmic and nuclear positivity in neuronal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human cerebral cortex shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human liver.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Analysis in human cerebral cortex and liver tissues. Corresponding NECAB1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human colon shows no positivity in glandular cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human cerebral cortex, colon, liver and pancreas using Anti-NECAB1 antibody NBP1-84003 (A) shows similar protein distribution across tissues to independent antibody NBP1-84004 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NECAB1 Antibody [NBP1-84003] - Staining of human liver shows no positivity in hepatocytes as expected.