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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-40357 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: PWKHAGKQDF REDQDAAFFK AWAIFKGKYK EGDTGGPAVW KTRLRCALNK
- Concentration
- 0.5 mg/mL
Submitted references Long Noncoding RNA H19 Participates in the Regulation of Adipose-Derived Stem Cells Cartilage Differentiation.
Pang HL, Zhao QQ, Ma Y, Song YL, Min J, Lu JR, Li H, Zhao DQ
Stem cells international 2019;2019:2139814
Stem cells international 2019;2019:2139814
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with IFN alpha (10ng/ml for 16 hr) (Lane 2), HeLa (Lane 3), HeLa treated with IFN alpha (10ng/ml for 16 hr) (Lane 4) and A549 (Lane 5). The blot was probed with Anti-IRF9 Polyclonal Antibody (Product # PA5-40357, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 43 kDa band corresponding to IRF9 was observed across the cell lines tested and was enhanced upon treatment.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-ISGF3G polyclonal antibody (Product # PA5-40357).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of IRF9 was achieved by transfecting HeLa with IRF9 specific siRNAs (Silencer® select Product # s20291, s20292). Western blot analysis (Fig. a) was performed using whole cell extracts from the IRF9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with IRF9 Polyclonal Antibody (Product # PA5-40357, 1 µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to IRF9. [Note: The knockdown of IRF9 siRNA is normalized to scrambled siRNA since studies show a feedback mechanism for upregulation.]
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human liver tissue using an anti-ISGF3G polyclonal antibody (Product # PA5-40357).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Validation of relative expression levels of representative DEGs in the osteoclast differentiation pathway. (a) Representative 9 miRNAs detected by RNA-Seq were confirmed using qRT-PCR. (b) The mRNA levels of STAT2 and IRF9 detected by RNA-Seq were confirmed using qRT-PCR. (c) Protein levels of STAT2 were confirmed using western blot. (d) Protein levels of IRF9 were confirmed using western blot ( * P < 0.05, ** P < 0.01, *** P < 0.001).
