PA5-34657

antibody from Invitrogen Antibodies

Targeting: APP AD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34657

Provider

Invitrogen Antibodies

Product name

Amyloid Precursor Protein Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, NIH-3T3, U87-MG, SK-N-SH, IMR32, SK-N-AS.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of beta Amyloid using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a beta Amyloid polyclonal antibody (Product # PA5-34657) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Amyloid Precursor Protein Polyclonal Antibody (Product # PA5-34657). Sample (30 µg of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. Amyloid Precursor Protein Polyclonal Antibody (Product # PA5-34657) diluted at 1:1500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of beta Amyloid was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a beta Amyloid Polyclonal Antibody (Product # PA5-34657) at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of beta Amyloid was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Amyloid Polyclonal Antibody (Product # PA5 34657) diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of beta Amyloid was performed in paraffin-embedded Rat brain tissue using beta Amyloid Polyclonal Antibody (Product # PA5-34657) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP (Product # PA5-34657) antibody (10 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.