- 710174 - Invitrogen Antibodies APP antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Sandwich ELISA was performed using Beta Amyloid 1-40 Recombinant Rabbit Polyclonal Antibody (Product # 710174) as detector in Aß 40 Human ELISA Kit (Invitrogen Product # KHB3481) following kit conditions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody was determined.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Beta Amyloid (1-40)showing staining in the cytoplasm and membrane of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Beta Amyloid (1-40)Recombinant Rabbit Polyclonal Antibody (Product # 710174) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Beta Amyloid (1-40)showing staining in the cytoplasm and membrane of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Beta Amyloid (1-40)Recombinant Rabbit Polyclonal Antibody (Product # 710174) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of beta-Amyloid in human brain tissue compared to a negative control using a beta-Amyloid Recombinant Rabbit Polyclonal Antibody (Product # 710174) followed by detection using an HRP-conjugated Goat anti-Rabbit IgG secondary antibody and DAB/H2O2. Tissues were rehydrated followed by endogenous peroxide blocking and heat-induced antigen retieval. Sections were counterstained with haematoxylin for nuclear staining. Staining is shown in the extracellular amyloid plaques (B). Magnified image of amyloid plaques (C).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Sandwich ELISA was performed using Beta Amyloid 1-40 Recombinant Rabbit Polyclonal Antibody (Product # 710174) as detector in Aß 40 Human ELISA Kit (Invitrogen Product # KHB3481) following kit conditions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody was determined.

710174