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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX48760 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48760, RRID:AB_11162848
- Product name
- Esrp-1/2 antibody [23A7.C9]
- Antibody type
- Monoclonal
- Antigen
- Other
- Host
- Mouse
- Vial size
- 50µg
- Storage
- Store vial at 4° C for short-term use. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: µgFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
- Validation comment
- WB
