- A15726 - Invitrogen Antibodies THY1 antibody

Submitted references Targeting MAD2 modulates stemness and tumorigenesis in human Gastric Cancer cell lines.

Pajuelo-Lozano N, Alcalá S, Sainz B Jr, Perona R, Sanchez-Perez I
Theranostics 2020;10(21):9601-9618

CD26 Identifies a Subpopulation of Fibroblasts that Produce the Majority of Collagen during Wound Healing in Human Skin.

Worthen CA, Cui Y, Orringer JS, Johnson TM, Voorhees JJ, Fisher GJ
The Journal of investigative dermatology 2020 Dec;140(12):2515-2524.e3

Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells.

Kempf H, Olmer R, Haase A, Franke A, Bolesani E, Schwanke K, Robles-Diaz D, Coffee M, Göhring G, Dräger G, Pötz O, Joos T, Martinez-Hackert E, Haverich A, Buettner FFR, Martin U, Zweigerdt R
Nature communications 2016 Dec 9;7:13602

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Separation of Jurkat cell line (red) from SP2 cell line (blue) in flow cytometry analysis (surface staining) stained using anti-human CD90 (5E10) APC Monoclonal antibody (Product # A15726).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Characterization of GCSCs. (A) Relative levels of mRNA transcripts for pluripotency and stemness markers. The relative expression levels of NANOG , SOX2 , OCT3 /4, LGR5 and LOXL2 mRNAs were quantified by RT-qPCR in MKN45, ST2957 and SNU638 cell lines cultured in adherence (ADH) or as spheres (CSCs). Each gene was normalized to GAPDH . Fold changes were calculated and compared to ADH (set as 1.0). At least three independent experiments were performed with similar results. (B) Flow cytometric analysis for CXCR4, CD44, CD90 and CD133 cell surface expression and autofluorescence (Fluo) in the 3 GC cell lines cultured in adherence (ADH) or as spheres (CSCs). The histograms summarize the percentage of CXCR4-, CD44-, CD90-, CD133- and autofluorescent-positive cells from three different experiments. Fold changes were calculated compared to ADH (set as 1.0). (C) ALDH activity profile. MKN45, ST2957 and SNU638 cell lines cultured in adherence (ADH) or as spheres (CSCs) and were counter-stained with AldeRed to determine the frequency of ALDH-positive cells by flow cytometry, in the presence or absence of the ALDH inhibitor (DEAB). ALDH activity is expressed as mean of the percentage of positive cells of 3 experiments. (D) Activation of signaling pathways in CSCs. Representative Western Blot analysis for GLI1, cleaved Notch1 and beta-catenin in the 3 GC cell lines cultured in adherent and sphere conditions. Graphs show the fold change of protein levels compared to ADH expression

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 MAD2 modulates pluripotency and stemness ability in GC cell lines. (A) The interference of MAD2L1 in MKN45 CSCs (shM) was confirmed by RT-qPCR. The relative expression levels of NANOG , SOX2 , OCT3 /4, LGR5 , CDH1 , VIM and LOXL2 mRNAs were quantified by RT-qPCR in the MKN45 CSCs . Each gene was normalized to GAPDH . Fold changes were calculated relative to the non-interfered CSCs (WT). At least, three independent experiments were performed with similar results. (B) Flow cytometric analysis of CXCR4, CD90 and CD133 cell surface markers in MKN45 CSCs after interference of MAD2L1 . The histograms summarize the percentage of CXCR4-, CD90- and CD133-positive cells in sphere cultures from three different experiments. Fold changes were calculated compared to WT. Representative plots with the percent-positive cells present within the single-cell, live and debris-free population are shown. (C) ALDH activity profile. MKN45 CSCs after interference of MAD2L1 were counter-stained with AldeRed to determine the frequency of ALDH-positive cells by flow cytometry, in the presence or absence of the ALDH inhibitor (DEAB). ALDH activity is expressed as mean of the percentage of positive cells of 3 experiments. Representative plots are shown, in the presence or absence of the ALDH inhibitor (DEAB), with the percent-positive cells present within the single-cell, live and debris-free population. (D) Secondary sphere formation efficiency in MKN45 CSCs with reduced MAD2 levels. 1 cell/well

A15726

Antibody data