Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB200-607-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Carbonic Anhydrase II/CA2 Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Carbonic Anhydrase II/CA2 Antibody [Biotin] [NB200-607] - Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 32 kDa for Carbonic Anhydrase II.