NBP2-48224

antibody from Novus Biologicals
Targeting: ZAP70 SRK, STD, ZAP-70
Provider product page for NBP2-48224

Antibody data

Product number
NBP2-48224 - Provider product page
Provider
Novus Biologicals
Product name
Mouse Monoclonal ZAP70 Antibody
Antibody type
Monoclonal
Description
Protein A or G purified. ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Reactivity
Human
Host
Mouse
Isotype
IgG
Vial size
7 ml
Storage
Store at 4C.
ZAP70 protein structure - NBP2-48224 shown in red.