AB7223-200

antibody from SICGEN

Targeting: RAB27A GS2, HsT18676, RAB27, RAM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AB7223-200

Provider

SICGEN

Proper citation

SICGEN Cat#AB7223-200, RRID:AB_2333182

Product name

Anti-Rab27

Antibody type

Polyclonal

Description

Goat polyclonal antibody to mouse Rab27. Rab27 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

600 µg

Concentration

3 mg/ml

Storage

Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

SICGEN (provider)

Main image

Experimental details

Cell lysates at 100 µg per lane

Sample type

Melan-Ink, AtT-20

Primary Ab dilution

1/500

Conjugate

Unconjugated

Secondary Ab

Secondary Ab

Secondary Ab dilution

1/10,000

Immunocytochemistry

Supportive validation

Submitted by

SICGEN (provider)

Main image

Experimental details

Cell were fixed with 4% PFA

Sample type

B6-RPE07

Primary Ab dilution

1/50

Conjugate

Unconjugated

Supportive validation

Submitted by

SICGEN (provider)

Main image

Experimental details

Cells were fixed with methanol

Sample type

Primary RPE cells

Primary Ab dilution

1/50

Immunohistochemistry

Supportive validation

Submitted by

SICGEN (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval;

Sample type

Human mammary tissue

Primary Ab dilution

1/200

Conjugate

Unconjugated