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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C354965 - Provider product page
- Provider
- LSBio
- Product name
- STAT1 Antibody (phospho-Tyr701) LS-C354965
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- WB using Phospho-STAT1 pTyr701 Antibody
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of STAT1 (pY701) showing staining in the cytoplasm and nucleus of paraffin-embedded human cervical carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a STAT1 (pY701) Rabbit Polyclonal Antibody diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
