PAB29354

antibody from Abnova Corporation

Targeting: STAT1 ISGF-3, STAT91

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29354

Provider

Abnova Corporation

Product name

STAT1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human STAT1.

Antigen sequence

LLRFSESSREGAITFTWVERSQNGGEPDFHAVEPY
TKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYP
NIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKT
ELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVG
SVE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with STAT1 polyclonal antibody (Cat # PAB29354) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with STAT1 polyclonal antibody (Cat # PAB29354) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human urinary bladder with STAT1 polyclonal antibody (Cat # PAB29354) shows moderate cytoplasmic and nuclear positivity in urothelial cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)