686402

antibody from BioLegend

Targeting: STAT1 ISGF-3, STAT91

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

686402

Provider

BioLegend

Proper citation

BioLegend Cat#686402, RRID:AB_2616883

Product name

Purified anti-STAT1 Phospho (Ser727)

Antibody type

Monoclonal

Antigen

Human STAT1 peptide phosphorylated at Ser 727.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A15158B

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total protein extract from HeLa cells untreated and treated with UV light for five minutes, from HeLa cells untreated and treated with 200ng/ml nocodazole overnight and from Baf3 cells untreated and treated with UV light for five minutes were resolved by electrophoresis (4-12% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-STAT1 Phospho (Ser 727)(clone A15158B) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (cat#405306) and chemiluminescence detection. Purified anti-β-actin antibody (clone Poly6221) was used as a loading control.

Conjugate

Unconjugated