SM7029P

antibody from Acris Antibodies GmbH

Targeting: DNMT3B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

SM7029P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SM7029P, RRID:AB_980475

Product name

anti DNMT3B

Antibody type

Monoclonal

Antigen

Bacteria expressed HIS-tag recombinant mouse Dnmt3b.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

52A1018

Vial size

0.1 mg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis for Dnmt3b using SM7029P at 2 µg/ml against 10 µg of cell lysates of 293 transfected with Mouse Dnmt3a (Lane 1) and Dnmt3b (Lane 2). A protein band of 110 kDa is only detected in 293 cells transfected with Mouse Dnmt3b cDNA.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of Dnmt3b in A) NIH 3T3 cell lysate, B) recombinant Dnmt3b1 protein and C) 2102Ep human embryonal carcinoma cell lysate using SM7029P DNMt3B antibody at 4 µg/ml, 0.5 µg/ml, and 3 µg/ml respectively.