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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-60654 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60654, RRID:AB_922080
- Product name
- Rabbit Polyclonal Nbs1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Interaction of FUS and HDAC1 regulates DNA damage response and repair in neurons.
SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.
Wang WY, Pan L, Su SC, Quinn EJ, Sasaki M, Jimenez JC, Mackenzie IR, Huang EJ, Tsai LH
Nature neuroscience 2013 Oct;16(10):1383-91
Nature neuroscience 2013 Oct;16(10):1383-91
SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.
Dobbin MM, Madabhushi R, Pan L, Chen Y, Kim D, Gao J, Ahanonu B, Pao PC, Qiu Y, Zhao Y, Tsai LH
Nature neuroscience 2013 Aug;16(8):1008-15
Nature neuroscience 2013 Aug;16(8):1008-15
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.
