Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-60648 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60648, RRID:AB_922083
- Product name
- Rabbit Polyclonal Nbs1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Role of mammalian Mre11 in classical and alternative nonhomologous end joining.
Xie A, Kwok A, Scully R
Nature structural & molecular biology 2009 Aug;16(8):814-8
Nature structural & molecular biology 2009 Aug;16(8):814-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nbs1 Antibody [NB100-60648] - NBS1 Antibody [NB100-60648] - Detection of Mouse NBS1 on HeLa whole cell lysate using NB100-60648. NBS1 was also immunoprecipitated by another rabbit anti-NBS1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] (lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Observed Molecular Weight at ~95 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Nbs1 Antibody [NB100-60648] - Detection of mouse NBS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from L1210 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NBS1 antibody NB100-60648 used for IP at 3 ug per reaction. NBS1 was also immunoprecipitated by rabbit anti-NBS1 antibody from Company B. For blotting immunoprecipitated NBS1, NB100-60648 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.