14-9934-82

antibody from Invitrogen Antibodies

Targeting: CASP8 Casp-8, FLICE, MACH, MCH5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9934-82

Provider

Invitrogen Antibodies

Product name

Caspase 8 Monoclonal Antibody (1H10), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody 1H10 recognizes human Caspase-8 also known as FLICE and MACH, a member of the cysteine-requiring aspartate protease family. Caspase-8 is an upstream caspase responsible for Fas and TNF-alpha mediated apoptosis. After activation of the Fas or TNF receptors by their ligands, the adaptor molecule FADD binds to the receptor and recruits caspase-8 to the membrane. The formation of this complex induces the cleavage and activation of caspase-8. The active form of caspase-8, made of a heterotetramer of p18 and p10, is released in the cytoplasm and activates downstream apoptotic proteases (caspase-3, -4, -6, -7, -9 and -10). Several (> 8) isoforms have been identified: 3 different pro-forms called Caspase-8a (55kDA), Caspase-8b (53kDa), and Caspase-8L which is procaspase-8 with an additional N-terminal extension of 59 aa.

Antibody clone number

1H10

Concentration

0.5 mg/mL

References

Submitted references

Caspase-8 is required for cell death induced by expanded polyglutamine repeats.

Sánchez I, Xu CJ, Juo P, Kakizaka A, Blenis J, Yuan J
Neuron 1999 Mar;22(3):623-33

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Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis.

Li H, Zhu H, Xu CJ, Yuan J
Cell 1998 Aug 21;94(4):491-501

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cell lysates nonreduced (lane 1) or reduced (lane 2) were resolved by SDS-PAGE then immunoblotted with 2 µg/mL of Anti-Human Caspase 8 Purified. Bands were visualized using Anti-Rat IgG HRP.