PA5-46808

antibody from Invitrogen Antibodies

Targeting: FADD GIG3, MORT1

Antibody data

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Validation data

Product number: PA5-46808 - Provider product page
Provider: Invitrogen Antibodies
Product name: FADD Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: AHLVGALRSC QMNLVADLVQ EVQQARDLQN RSGAMSPMSW NSDASTSEAS
Concentration: 0.5 mg/mL

FADD protein structure - PA5-46808 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of human thymus cells using an anti-FADD polyclonal antibody (Product # PA5-46808).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of FADD was achieved by transfecting MDA-MB-231 Cells with FADD specific siRNAs (Silencer® select Product # s16705, s16706). Western blot analysis (Fig. a) was performed using whole cell extracts from the FADD knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FADD Polyclonal Antibody (Product # PA5-46808, 1 µg/mL) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FADD.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-FADD Polyclonal Antibody (Product # PA5-46808) and a band at ~23 kDa band corresponding to FADD was observed across the cell lines tested. An additional uncharacterized band (*) was observed in A-431. Whole cell extracts (30 ug lysate) of A-431 (Lane 1), A549 (Lane 2), HT-29 (Lane 3) and MDA-MB-231 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).