Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-46808 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FADD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: AHLVGALRSC QMNLVADLVQ EVQQARDLQN RSGAMSPMSW NSDASTSEAS
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human thymus cells using an anti-FADD polyclonal antibody (Product # PA5-46808).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of FADD was achieved by transfecting MDA-MB-231 Cells with FADD specific siRNAs (Silencer® select Product # s16705, s16706). Western blot analysis (Fig. a) was performed using whole cell extracts from the FADD knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FADD Polyclonal Antibody (Product # PA5-46808, 1 µg/mL) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FADD.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-FADD Polyclonal Antibody (Product # PA5-46808) and a band at ~23 kDa band corresponding to FADD was observed across the cell lines tested. An additional uncharacterized band (*) was observed in A-431. Whole cell extracts (30 ug lysate) of A-431 (Lane 1), A549 (Lane 2), HT-29 (Lane 3) and MDA-MB-231 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human kidney tissue using an anti-FADD polyclonal antibody (Product # PA5-46808).